Review



pgp cmvgcamp6s  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Addgene inc pgp cmvgcamp6s
    Pgp Cmvgcamp6s, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 298 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gcamp6s+plasmid/pGP-CMV-GCaMP6s+(Plasmid+%2340753)/pm41915748-388-14-15
    Average 95 stars, based on 298 article reviews
    pgp cmvgcamp6s - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: Advancing Genetic-based Tools in Insect Vectors of Disease
    Article Snippet: .. To generate the GCaMP6s plasmid (plasmid sequence and DNA available for order at addgene ID# 106868), components were cloned into the piggyBac plasmid pBac-3xP3-dsRed (Li et al. 2017) using Gibson assembly/EA cloning (Gibson et al. 2009). ..

    Article Title: Coupling-dependent metabolic ultradian rhythms in confluent cells
    Article Snippet: For the CMV promoter–driven luciferase plasmid (pLV_CMV-Luc-ires-Hygr), the CMV promoter was PCR amplified from a pcDNA3.1 vector, and luciferase and hygromycin resistance genes were amplified from pGL4.31 and separated by an internal ribosome entry site sequence. .. For the CMV promoter–driven GCaMP6s plasmid, the E2Luc cassette in pLV_E2Luc-Blst was replaced with the CMV promoter–driven GCaMP6s amplified from pGP-CMV-GCaMP6s (a gift from Douglas Kim; Addgene plasmid No. 40753) ( ). .. Lentiviral vectors coding for short hairpin RNAs (shRNAs) targeting human gap junction genes Cx43 and Cx45 were gifts from Dr. Jerry Shay at University of Texas Southwestern Medical Center. psPAX2 and pMD2.G were gifts from Didier Trono, Addgene (plasmid No. 12260 and No. 12259) and were used to package the lentivirus.

    Article Title: Pyrimidinergic calcium signaling links tubular metabolism to fibrosis in kidney disease.
    Article Snippet: .. Thereafter, transfection with GCaMP6s plasmid (pGP-CMV-GCaMP6s, #40753, Addgene) followed. .. After 48h cells were perfused with 10 μM P2Y6R agonist (MRS2957) and changes in fluorescence intensity were detected by an inverted confocal laser scanning microscope ZEISS LSM 980 Airyscan by using a LD LCI Plan-Apochromat 25x/0.8 water objective.

    Article Title: Pyrimidinergic calcium signaling links tubular metabolism to fibrosis in kidney disease
    Article Snippet: Imaging was performed on an inverted confocal laser scanning microscope ZEISS LSM 980 Airyscan by using an LD LCI Plan-Apochromat 25×/0.8 silicon-oil objective. .. NRK-49F cells were grown in 8-well chamber slides for 72 h. Thereafter, transfection with the GCaMP6s plasmid (pGP-CMV-GCaMP6s, #40753, Addgene) followed. .. After 48 h, cells were perfused with 10 μM P2Y6R agonist (MRS2957) and changes in fluorescence intensity were detected by an inverted confocal laser scanning microscope ZEISS LSM 980 Airyscan by using an LD LCI Plan-Apochromat 25x/0.8 water objective.

    Article Title: Coupling-dependent metabolic ultradian rhythms in confluent cells.
    Article Snippet: For the CMV promoter–driven luciferase plasmid (pLV_CMV-Luc-ires-Hygr), the CMV promoter was PCR amplified from a pcDNA3.1 vector, and luciferase and hygromycin resistance genes were amplified from pGL4.31 and separated by an internal ribosome entry site sequence. .. For the CMV promoter–driven GCaMP6s plasmid, the E2Luc cassette in pLV_E2Luc-Blst was replaced with the CMV promoter–driven GCaMP6s amplified from pGP-CMV-GCaMP6s (a gift from Douglas Kim; Addgene plasmid No. 40753) (49). .. Lentiviral vectors coding for short hairpin RNAs (shRNAs) targeting human gap junction genes Cx43 and Cx45 were gifts from Dr. Jerry Shay at University of Texas Southwestern Medical Center. psPAX2 and pMD2.G were gifts from Didier Trono, Addgene (plasmid No. 12260 and No. 12259) and were used to package the lentivirus.

    Sequencing:

    Article Title: Advancing Genetic-based Tools in Insect Vectors of Disease
    Article Snippet: .. To generate the GCaMP6s plasmid (plasmid sequence and DNA available for order at addgene ID# 106868), components were cloned into the piggyBac plasmid pBac-3xP3-dsRed (Li et al. 2017) using Gibson assembly/EA cloning (Gibson et al. 2009). ..

    Clone Assay:

    Article Title: Advancing Genetic-based Tools in Insect Vectors of Disease
    Article Snippet: .. To generate the GCaMP6s plasmid (plasmid sequence and DNA available for order at addgene ID# 106868), components were cloned into the piggyBac plasmid pBac-3xP3-dsRed (Li et al. 2017) using Gibson assembly/EA cloning (Gibson et al. 2009). ..

    Cloning:

    Article Title: Advancing Genetic-based Tools in Insect Vectors of Disease
    Article Snippet: .. To generate the GCaMP6s plasmid (plasmid sequence and DNA available for order at addgene ID# 106868), components were cloned into the piggyBac plasmid pBac-3xP3-dsRed (Li et al. 2017) using Gibson assembly/EA cloning (Gibson et al. 2009). ..

    other:

    Article Title: VAP spatially stabilizes dendritic mitochondria to locally support synaptic plasticity
    Article Snippet: The Fis1-Lifeact-GFP, Fis1-mCherry, and Spire1 KD plasmids were obtained from the Manor Lab, Mff-OE and Srgap2 KD plasmids from the Polleux Lab, VAP KD and Control plasmids from the Hoogenraad Lab, VAP KO and Control sgRNA plasmids from the Hoppa Lab, Vapb-emerald and Vapb-HaloTag plasmids from the Lippincott-Schwartz Lab, Inf2 KD plasmid from the Higgs Lab, ER-VSVG-GFP and PSD95-mCherry plasmids from Hanus C, and Homer2-mOrange plasmid from the de Juan-Sanz Lab. Lifeact-mCherry plasmid (gift from Michael Davidson), Mito-BFP plasmid, Drp1 KD plasmid (gift from David Kashatus), Snca KD and Control plasmids, Mito-PAGFP plasmid, and GCaMP6s plasmid were purchased from Addgene (plasmids 54491, 49151, 99385, 75437, 75438, 23348, 40753, respectively).

    Article Title: Activity-dependent regulation of mitochondrial motility in developing cortical dendrites
    Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus, male/ female) C57Bl/6 J, male and female Janvier Labs; Transfected construct GCaMP6s (species: Rattus norvegicus) Add gene plasmid 40753; Douglas Kim RRID:Addgene_40753 Cloned into pCAG vector Transfected construct Mitochondrial- DsRed (species: Homo sapiens) Gift from Thomas Misgeld Mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase causing mitochondrial localization as previously described; Rizzuto et al., 1995; Li et al., 2004; MacAskill et al., 2009 Cloned into pCAG vector Chemical compound, drug TTX 1078, Bio- Techne, Minneapolis, MN Chemical compound, drug LTX ALX- 630–027 C040, Enzo Life Sciences b.v., Farmingdale, NY Chemical compound, drug Glutamate G1626, Sigma Software, algorithm MitoMotil This study https:// github. com/ annikc/ MitoMotil (copy archived at swh:1:rev:a4cfb2b4fd66579f63ea5a150a0f9b1b21b89a83, Yalnizyan- Carson, 2021) Software, algorithm MATLAB The MathWorks https:// mathworks. com Software, algorithm NormCorre Flatiron Institute, Simons Foundation https:// github. com/ flatironinstitute/ NoRMCorre, Pnevmatikakis and Giovannucci, 2021 Software, algorithm Python Python Software Foundation https://www. python. org/ Software, algorithm Elephant library Human Brain Project https:// elephant. readthedocs. io/ en/ latest/

    Amplification:

    Article Title: Coupling-dependent metabolic ultradian rhythms in confluent cells
    Article Snippet: For the CMV promoter–driven luciferase plasmid (pLV_CMV-Luc-ires-Hygr), the CMV promoter was PCR amplified from a pcDNA3.1 vector, and luciferase and hygromycin resistance genes were amplified from pGL4.31 and separated by an internal ribosome entry site sequence. .. For the CMV promoter–driven GCaMP6s plasmid, the E2Luc cassette in pLV_E2Luc-Blst was replaced with the CMV promoter–driven GCaMP6s amplified from pGP-CMV-GCaMP6s (a gift from Douglas Kim; Addgene plasmid No. 40753) ( ). .. Lentiviral vectors coding for short hairpin RNAs (shRNAs) targeting human gap junction genes Cx43 and Cx45 were gifts from Dr. Jerry Shay at University of Texas Southwestern Medical Center. psPAX2 and pMD2.G were gifts from Didier Trono, Addgene (plasmid No. 12260 and No. 12259) and were used to package the lentivirus.

    Article Title: Coupling-dependent metabolic ultradian rhythms in confluent cells.
    Article Snippet: For the CMV promoter–driven luciferase plasmid (pLV_CMV-Luc-ires-Hygr), the CMV promoter was PCR amplified from a pcDNA3.1 vector, and luciferase and hygromycin resistance genes were amplified from pGL4.31 and separated by an internal ribosome entry site sequence. .. For the CMV promoter–driven GCaMP6s plasmid, the E2Luc cassette in pLV_E2Luc-Blst was replaced with the CMV promoter–driven GCaMP6s amplified from pGP-CMV-GCaMP6s (a gift from Douglas Kim; Addgene plasmid No. 40753) (49). .. Lentiviral vectors coding for short hairpin RNAs (shRNAs) targeting human gap junction genes Cx43 and Cx45 were gifts from Dr. Jerry Shay at University of Texas Southwestern Medical Center. psPAX2 and pMD2.G were gifts from Didier Trono, Addgene (plasmid No. 12260 and No. 12259) and were used to package the lentivirus.

    Transfection:

    Article Title: Pyrimidinergic calcium signaling links tubular metabolism to fibrosis in kidney disease.
    Article Snippet: .. Thereafter, transfection with GCaMP6s plasmid (pGP-CMV-GCaMP6s, #40753, Addgene) followed. .. After 48h cells were perfused with 10 μM P2Y6R agonist (MRS2957) and changes in fluorescence intensity were detected by an inverted confocal laser scanning microscope ZEISS LSM 980 Airyscan by using a LD LCI Plan-Apochromat 25x/0.8 water objective.

    Article Title: Pyrimidinergic calcium signaling links tubular metabolism to fibrosis in kidney disease
    Article Snippet: Imaging was performed on an inverted confocal laser scanning microscope ZEISS LSM 980 Airyscan by using an LD LCI Plan-Apochromat 25×/0.8 silicon-oil objective. .. NRK-49F cells were grown in 8-well chamber slides for 72 h. Thereafter, transfection with the GCaMP6s plasmid (pGP-CMV-GCaMP6s, #40753, Addgene) followed. .. After 48 h, cells were perfused with 10 μM P2Y6R agonist (MRS2957) and changes in fluorescence intensity were detected by an inverted confocal laser scanning microscope ZEISS LSM 980 Airyscan by using an LD LCI Plan-Apochromat 25x/0.8 water objective.



    Similar Products

    95
    Addgene inc pgp cmvgcamp6s
    Pgp Cmvgcamp6s, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gcamp6s+plasmid/pGP-CMV-GCaMP6s+(Plasmid+%2340753)/pm41915748-388-14-15
    Average 95 stars, based on 1 article reviews
    pgp cmvgcamp6s - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    96
    Addgene inc paav syn flex gcamp6s wpre sv40 aav9
    Paav Syn Flex Gcamp6s Wpre Sv40 Aav9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gcamp6s+plasmid/pAAV%2ESyn%2EFlex%2EGCaMP6s%2EWPRE%2ESV40+(Plasmid+%23100845)/pm41902755-71-17-19
    Average 96 stars, based on 1 article reviews
    paav syn flex gcamp6s wpre sv40 aav9 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    93
    Addgene inc aav1 hsynapsin1 axon gcamp6s
    Aav1 Hsynapsin1 Axon Gcamp6s, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gcamp6s+plasmid/pAAV-hSynapsin1-axon-GCaMP6s+(Plasmid+%23111262)/pm41896306-588-31-37
    Average 93 stars, based on 1 article reviews
    aav1 hsynapsin1 axon gcamp6s - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    96
    Addgene inc aav gcamp6s wpre sv40
    Aav Gcamp6s Wpre Sv40, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gcamp6s+plasmid/pAAV%2ESyn%2EGCaMP6s%2EWPRE%2ESV40+(Plasmid+%23100843)/pm41892247-84-4-5
    Average 96 stars, based on 1 article reviews
    aav gcamp6s wpre sv40 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Addgene inc sv40
    Sv40, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gcamp6s+plasmid/pAAV%2ESyn%2EGCaMP6s%2EWPRE%2ESV40+(Plasmid+%23100843)/pm41896306-591-0-6
    Average 96 stars, based on 1 article reviews
    sv40 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    93
    Addgene inc addgene plasmid no 111262
    Addgene Plasmid No 111262, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gcamp6s+plasmid/pAAV-hSynapsin1-axon-GCaMP6s+(Plasmid+%23111262)/pm41896306-586-41-41
    Average 93 stars, based on 1 article reviews
    addgene plasmid no 111262 - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    96
    Addgene inc aav9
    Aav9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gcamp6s+plasmid/pAAV%2ESyn%2EGCaMP6s%2EWPRE%2ESV40+(Plasmid+%23100843)/bio_rxiv__64898__2026__03__26__713939-219-25-21
    Average 96 stars, based on 1 article reviews
    aav9 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Addgene inc aav syn gcamp6s wpre sv40
    Aav Syn Gcamp6s Wpre Sv40, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gcamp6s+plasmid/pAAV%2ESyn%2EGCaMP6s%2EWPRE%2ESV40+(Plasmid+%23100843)/bio_rxiv__64898__2026__03__26__713939-219-23-21
    Average 96 stars, based on 1 article reviews
    aav syn gcamp6s wpre sv40 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    94
    Addgene inc paav cag gcamp6s
    Paav Cag Gcamp6s, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gcamp6s+plasmid/pAAV%2ECAG%2EGCaMP6s%2EWPRE%2ESV40+(Plasmid+%23100844)/pmc12969040-42-0-2
    Average 94 stars, based on 1 article reviews
    paav cag gcamp6s - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    Image Search Results